QUANTITATIVE ANALYSIS OF PAMABROM AND IBUPROFEN IN SYNTHETIC MIXTURE USING 1 ST ORDER DERIVATIVE SPECTROSCOPY
By: Patel, Pinak
.
Contributor(s): Vasani, Priyanka.
Publisher: M P Innovare Academic Sciences Pvt Ltd 2019Edition: Vol.11(10).Description: 26-32p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
International journal of pharmacy and pharmaceutical scienceSummary: Objective:
The preliminary goal was to develop and validate 1
st
order derivative spectroscopic method for quantita
tive analysis of Pamabrom
(PAMA) which is a xanthine diuretic and ibuprofen (
IBU) which is a non-steroidal anti-inflammatory age
nt from its synthetic mixture.
Methods:
Analytical method was developed on Shimadzu double
beam spectrophotometer equipped with UV probe 2.42
as software using
methanol as solvent. Quantification of PAMA was car
ried out at zero cross over point of IBU that is 29
1 nm and for IBU, it was achieved at 278 nm
which is zero cross over point of PAMA. Method was
validated according to ICH Q2 R1 guidelines.
Results:
Method showed a linear response in the range of 2-1
2 μg/ml of PAMA and 20-120 μg/ml of IBU. Method was
found to be accurate with recovery
between 99.7–100.9 % for PAMA and 100.3–100.7 % for
IBU. The method was found to be accurate and preci
se for quantitative analysis of PAMA and IBU.
Conclusion:
The developed method was successfully validated as
per ICH Q2 R1 guidelines and was successfully appli
ed for quantitative analysis of
a synthetic mixture of PAMA and IBU.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2020861 |
Objective:
The preliminary goal was to develop and validate 1
st
order derivative spectroscopic method for quantita
tive analysis of Pamabrom
(PAMA) which is a xanthine diuretic and ibuprofen (
IBU) which is a non-steroidal anti-inflammatory age
nt from its synthetic mixture.
Methods:
Analytical method was developed on Shimadzu double
beam spectrophotometer equipped with UV probe 2.42
as software using
methanol as solvent. Quantification of PAMA was car
ried out at zero cross over point of IBU that is 29
1 nm and for IBU, it was achieved at 278 nm
which is zero cross over point of PAMA. Method was
validated according to ICH Q2 R1 guidelines.
Results:
Method showed a linear response in the range of 2-1
2 μg/ml of PAMA and 20-120 μg/ml of IBU. Method was
found to be accurate with recovery
between 99.7–100.9 % for PAMA and 100.3–100.7 % for
IBU. The method was found to be accurate and preci
se for quantitative analysis of PAMA and IBU.
Conclusion:
The developed method was successfully validated as
per ICH Q2 R1 guidelines and was successfully appli
ed for quantitative analysis of
a synthetic mixture of PAMA and IBU.
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